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Experimental electrophysiology

Electrophysiological cellular investigations of the neurogliovascular unit could be investigated on animal models of neurological diseases. Two electrophysiology platforms are located in the Alzheimer & Tauopathies, UMR 837 Inserm (Dr Luc Buée) and in the Alzheimer’s disease and Vascular Pathologies EA1046 (Prof Régis Bordet) departments. Electrophysiological investigations on isolated cells (freshly dissociated or cultured) from neuronal or vascular origins with different patch-clamp configurations or synaptic plasticity with LTP and LTD investigations on mouse or rat brain slices as well as neuronal ionic current recordings in brain slices (Dr S. Humez UMR 837, Dr M. Bastide EA1046) could be conducted.

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Whole-cell patch-clamp setup for current recordings on  isolated cells investigations


LTP, LTD and ionic currents measurements on brain slices

Bastide M, Ouk T, Plaisier F, Petrault O, Stolc S, Bordet R. Neurogliovascular unit after cerebral ischemia: Is the vascular wall a pharmacological target. Psychoneuroendocrinology. 2007;32 Suppl 1:S36-39

Clasadonte J, Poulain P, Hanchate NK, Corfas G, Ojeda SR, Prevot V. Prostaglandin E2 release from astrocytes triggers gonadotropin-releasing hormone (GnRH) neuron firing via EP2  receptor activation. Proc Natl Acad Sci U S A. 2011; 108(38):16104-9.

Plaisier F, Bastide M, Ouk T, Petrault O, Laprais M, Stolc S, Bordet R. Stobadine-induced hastening of sensorimotor recovery after focal ischemia/reperfusion is associated with cerebrovascular protection. Brain Res. 2008;1208:240-249

Van der Jeugd A, Ahmed T, Burnouf S, Belarbi K, Hamdame M, Grosjean ME, Humez S, Balschun D, Blum D, Buée L, D'Hooge R. Hippocampal tauopathy in tau transgenic mice coincides with impaired hippocampus-dependent learning and memory, and attenuated late-phase long-term depression of synaptic transmission. Neurobiol Learn Mem. 2011;95(3):296-304.